Needles Disinfection
Needles Disinfection about sterilization and disinfection? 1. What is the hazard of the splattering tendency in sterilizing a wire loop over a flame? 2. Can wire loops and needles be sterilized by...
Needles Disinfection
|
about sterilization and disinfection? 1. What is the hazard of the splattering tendency in sterilizing a wire loop over a flame? 2. Can wire loops and needles be sterilized by autoclaving? 3. Under what conditions is sterilization by filtration preffered over sterilization by heat? 4. Can culture media be sterilized in the hot air sterilizer? why or why not? 5. Why is heat an effective sterilizing agent? 6. Why is time of exposure and temperature required for sterilization greater for dry heat(160-180 C for 1-2 h0 than for autoclaving(121 C for 15 mins)? 7. What is the meaning of minimum inhibitory concentration? 1. Loops can hold moisture in small droplets. Placing them directly in the flame while wet can cause the moisture to turn to steam and spatter hot liquid onto unprotected face/skin. 2. Yes 3. When the liquid you need sterilized contains something that is heat labile (meaning it breaks down if it gets too hot), you would need to filter it, as opposed to heating it. 4. Hot air sterilizers would cause liquid media to boil and evaporate, destroying all of your hard work! 5. Heat denatures the proteins of contaminating microbes. Without those proteins, microbes cannot maintain structure or run biochemical reactions. 6. Many microbes have forms that are heat-stabile and can actually come back to life after being dried out. Autoclaving uses a lot of pressure along with heat to break through that protection and kill the cells once and for all. 7. The lowest concentration of a substance (e.g. bleach) needed to prevent the growth of a microorganism. This depends both on the organism as well as the type of antibacterial substance. |
||
|
No items matching your keywords were found. |
||
How Do I Inject and Procedure?Injecting oil based steroids (deca durabolin, masteron, primobolan, sustanon, testosterone enathate) is done with intramuscular needle (1,5 inch long), while water based steroids testosterone suspension and winstrol depot) are injection with smaller and shorter subskin needle (0,5 inch long). What to look for before injecting. Check the expiry dates of every product. Make sure that the vial or ampoule contains the right drug in the right strength. During the whole preparation procedure, material should be kept sterile. Wash your hands before starting to prepare the injection. Disinfect the skin over the injection site. Make sure that there are no air bubbles left in the syringe. Once the protective cover of the needle is removed extra care is needed. Do not touch anything with the unprotected needle. Once the injection has been given take care not to prick yourself or somebody else. Step by step for vials Wash your hands. Disinfect the top of the vial. Use a syringe with a volume of twice the required amount of liquid or solution and add the needle. Suck up as much air as the amount of solution needed to aspirate. Insert needle into (top of) vial and turn upside down. Pump air into vial (creating pressure). Aspirate the required amount of solution and 0.1 ml extra. Make sure the tip of the needle is below the fluid surface. Pull the needle out of the vial. Remove possible air from the syringe. Clean up; dispose of waste safely; wash your hands. Step by step for ampoules Wash your hands. Put the needle on the syringe. Remove the liquid from the neck of the ampoule by flicking it or swinging it fast in a downward spiraling movement. File around the neck of the ampoule. Protect your fingers with gauze if ampoule is made of glass. Carefully break off the top of the ampoule (for a plastic ampoule twist the top). Aspirate the fluid from the ampoule. Remove any air from the syringe. Clean up; dispose of working needle safely; wash your hands. Injecting Wash your hands. Reassure yourself / patient's for procedure. Uncover the area to be injected (lateral upper quadrant major gluteal muscle, lateral side of upper leg, deltoid muscle). Disinfect the skin. Relax the muscle. Insert the needle swiftly at an angle of 90 degrees (watch depth!). Aspirate briefly; if blood appears, withdraw needle. Replace it with a new one, if possible, and start again from point 4. Inject slowly (less painful). Withdraw needle swiftly. Press sterile cotton wool onto the opening. Fix with adhesive tape. Check yourself / patient's reaction and give additional reassurance, if necessary. Clean up; dispose of waste safely; wash your hands. About the Author Mark Henry is the author related to Order Anabolic Steroids, Deca Durabolin, Sustanon, Dianabol, Winstrol, Anadrol shopOrtpharmacy is an online anabolic steroids shop. In our shop purchase mail order steroids like deca durabolin, dianabol, sustanon250, winstrol depot, anadrol, testosterone mail order steroids, free bodybuilding steroids |